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VERSA Next Generation Library Prep (NGLP) Workstation



Next generation sequencing (NGS) technologies are emerging as a powerful means for providing gigabases of genetic information in a single run. The increase in throughput and reduction in sequencing set up costs have revolutionized genomic research. Furthermore, the application of these technologies has greatly expanded the ability of researchers to use genomic information for diverse applications. These include primary research, health screening, personalized medicine, and single nucleotide polymorphism characterization to name a few.

As the traditional bottlenecks associated with sequencing set up are relaxed, new demands are placed on sample preparation to replenish sequencing project pipelines. However, the labour-intensive and error-prone steps needed for sequencing set up make it difficult to produce large numbers of libraries.

Researchers at Aurora recognize that automation of this manual process maximizes the benefits offered by next generation sequencing (NGS), and thus have developed the VERSA Next Generation Library Prep (NGLP) Workstation. The VERSA NGLP Workstation for next generation sequencing library prep allows researchers to automate protocols involving DNA isolation, enzyme reaction setup and magnetic bead-based DNA fragment size selection. The VERSA Next Generation Library Prep Workstation is compatible with various commercially available kits and reagents, allowing researchers to reduce costs associated with library preparation. By providing accurate liquid handling, robust temperature control and consistent reliability, the VERSA Next Generation Library Prep Workstation ensures users get the most out of expensive samples and reagents.

Next generation library preparation

VERSA 110 NGLP

Sequencing Set Up

VERSA 1100 NGLP

Key Points

  • Walk-away library preparation for next generation sequencing set up technologies
  • Automates nucleic acid extraction, reverse transcription, and PCR setup
  • Compatible with 3rd party kits and reagents to reduce your costs
  • Scalable solution that can process up to 96 samples in parallel
  • Complete next generation sequencing library prep

VERSA Next Generation Library Prep (NGLP) Workstation Features

  • Single channel or 8-channel liquid handling module: Handle any throughput
  • Liquid level sensing: Reduce dead volumes, and maximize recovery of samples through cleanups
  • Reagent dispensing pins: Reduce both tip consumption and preparation time
  • Temperature control blocks: Fast ramping and precise temperature control for efficient enzymatic modification to your samples
  • Small footprint: Fit the workstation into a hood, or outfit it with its own UV / HEPA filtered hood

Resources

Publications


Improving NGS Library Prep with an Open System Liquid Handler

Growing next generation sequencing (NGS) throughput and new, sophisticated multiplexing strategies have significantly increased the number of samples users can prepare. The demand for reproducible and reliable data requires consistency between libraries. To achieve this, the VERSA Mini (110) NGLP, liquid handling workstation has been optimized for NGS library preparation. Using NEXTflex™ library preparation technology in conjunction with the VERSA Mini NGLP, high throughput functionality matched with superior enzyme performance outpaces and improves consistency when compared to manual library preparation. Read more...

Posters


Validation of VERSA Mini NGLP for Automated Library Preparation with the Illumina Platform

Plant and Animal Genome 2012, Pittcon 2012

The plummeting cost of sequence acquisition that has resulted from advances in next generation sequencing (NGS) technologies has led to its use in new settings such as small research groups, contract research organizations, and the clinic. Widespread adoption of NGS is quickly approaching, in spite of an underdeveloped field of liquid handling workstations validated to complete the associated library preparation protocols. As this task requires a high number of sample manipulations, consistently reliable results depend on liquid handling automation. download here...(pdf file 1.9MB)

For more information, please review the VERSA Next Generation Library Prep (NGLP) Workstation brochure or contact us directly to discuss your specific application. Thank you for considering AURORA to solve your lab automation requirements.

Download the VERSA Next Generation Library Prep (NGLP) Workstation Brochure:

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