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Automation of Reverse Transcription of Avian Influenza Viral RNA and Detection of H5N1 with VERSA 110 NAP/PCR Setup Workstation

automated nucleic acid preparation

The importance of rapid avian flu detection worldwide can not be understated. Recent advances in PCR and RT-PCR have accelerated the screening procedure, but sample preparation has lagged behind. Aurora Instruments addressed this problem by validating the VERSA 110 NAP / PCR Setup Workstation for automating the sample preparation steps involved in viral RNA detection from influenza A/H5N1 using a commercially available kit. The detection of amplified products of the proper size for the avian flu genes suggest that the VERSA workstation can be successfully used to automate sample preparation for the detection of a/H5N1 genes.

The VERSA 110 NAP / PCR Workstation, when housed in an appropriate bio-safety cabinet can be utilized to handle BSL-2 and BSL-3 agents reducing the risk of iatrogenic infections in laboratory workers.

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