Automated Liquid Handling
Automated Liquid Handling

Mycoplasma Pneumonia Diagnostic Kit (PCR-Fluorescence Probing)

Mycoplasma pneumonia is an atypical pneumonia in humans, caused by the bacterium Mycoplasma pneumoniae, resulting in immense irritation of respiratory systems. These infections have a relatively slow progression of symptoms. Blood tests require a minimum 10 day infection period, while ELISA tests suffer from interference resulting in high false-positive diagnoses. The Mycoplasma Pneumonia diagnostic test kit is designed for in vitro quantification of the pathogen. Detailed clinical study has validated the high specificity, sensitivity, and reproducibility of the kit with analytical sensitivity of 1000 copies/mL in the sample. The kit contains reagents for nucleic acid extraction, realtime-PCR detection and quality control.

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Catalogue #: ABI - Mycoplasma Pneumonia PCR Diagnostic Kit

Principles of the test

The kit has specifically designed primers combined with composite fluorescent probes, thereby allowing utilization of realtime fluorescent PCR. The genetic sequence of M. pneumoniae can be targeted through realtime-PCR amplification. Probes are double-stranded oligonucleotides consisting of two (long and short) complementary strands bound to the reporter and quencher probes, respectively. Variation in the fluorescent signal is directly proportional to the quantity of amplified product.

Key Features

The kit contains ready-to-use reagents, offers fast real-time detection and provides excellent sensitivity and high specificity. It is also optimized for use in automated liquid handling workstations. By combining this kit with Aurora’s VERSA™ workstations, laboratories can fully automate the amplification process thereby reducing cost and operator error, while increasing efficiency.

Product Features:

  • 24 tests per kit (25µL reaction)
  • Rapid detection of M. pneumoniae
  • All necessary PCR reagents pre-mixed and ready-to-go
  • Applicable for most commercially available realtime PCR instruments
  • Standard instrument set-up parameters
  • Accurate controls to confirm findings
  • Colour coded reagent vials

Standard Kit Contents:

  • DNA extraction reagent
  • PCR detection reagent
    • PCR Buffer
    • Primer/Probe/dNTPs
    • Taq polymerase
  • MgCl2
  • Quality control reagent
    • M. pneumoniae positive control
    • M. pneumoniae quantification reference
    • Negative control
  • Diluent

Dynamic range of kit

Under optimal PCR conditions the kit has very high priming efficiencies of >95% and can detect between 1X108 and 1X103 copies of target template.

Handling & Storage Guidelines

Kit Storage & Shelf life - 12 months at -20ºC±5ºC. Repeated freezing and thawing should be avoided.

Sample Requirements & Capabilities

Sputum and throat swab

Sample collection, storage and transportation

Please follow the current guidelines and ordinance on M. pneumoniae specimen collection, handling, storage, packaging, transport requirements.

Disclaimer: The kit is for detection of the M. pneumoniae in the sputum, throat swabs, and the respiratory tract samples for the laboratory diagnosis and monitoring the particular infection. The kit is for research only, and should not be used in clinical diagnosis.

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