Automated Liquid Handling
Automated Liquid Handling

Mycobacterium Tuberculosis Diagnostic Kit (PCR-Fluorescence Probing)

Mycobacterium tuberculosis (MTB) is a bacterial pathogen that thrives in highly aerobic environments such as the human respiratory system. MTB infects the lungs, causing tuberculosis, which often results in serious or even lethal side-effects. The MTB diagnostic test kit is designed for in vitro quantification of these pathogens with high sensitivity. Detailed clinical study has validated the high specificity, sensitivity, and reproducibility of the kit with analytical sensitivity of 1000 copies/mL in the sample. The kit contains reagents for nucleic acid extraction, realtime-PCR detection and quality control.

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Catalogue #: ABI - Mycobacterium Tuberculosis Diagnostic Kit

Principles of the test

The kit has specifically designed primers as well as a composite fluorescent probe thereby allowing utilization of realtime fluorescent PCR. The genetic sequence of MTB can be targeted through realtime-PCR amplification.

Key Features

The kit contains ready-to-use reagents, offers fast real-time detection and provides excellent sensitivity and high specificity. It is also optimized for use in automated liquid handling workstations. By combining this kit with Aurora’s VERSA™ workstations, laboratories can fully automate the amplification process thereby reducing cost and operator error, while increasing efficiency.

Product Features:

  • 48 tests per kit (25 µL reaction)
  • Rapid detection of M. tuberculosis.
  • All necessary PCR reagents pre-mixed and ready-to-go
  • Applicable for most commercially available realtime PCR instruments
  • High sensitivity and high precision in determining virus types
  • Standard instrument set-up parameters
  • Accurate controls to confirm findings
  • Colour coded reagent vials

Standard Kit Contents:

  • DNA extraction reagent
  • PCR detection reagent
    • PCR Buffer
    • Primer/Probe/dNTPs
    • Taq mixture
  • Quality control reagent
    • M. tuberculosis positive control
    • M. tuberculosis quantification reference
    • Negative control
  • Diluent

Dynamic range of kit

Under optimal PCR conditions the kit has very high priming efficiencies of >95% and can detect between 1X108 and 1X103 copies of target template.

Handling & Storage Guidelines

Kit Storage & Shelf life - Store at -20ºC±5ºC. Avoid repeated freezing and thawing. Kit is stable until expiry date shown.

Sample Requirements & Capabilities

This test should only be used for sputum samples.

Sample collection, storage and transportation

Please follow the current guidelines and ordinance on the Chlamydia trachomatis specimen collection, handling, storage, packaging, transport requirements.

Disclaimer: The kit is for detection of the Mycobacterium tuberculosis in endocervical and urethral swab specimens and may be used for the laboratory diagnosis and monitoring the particular virus infection. The kit is for research only, and should not be used in clinical diagnosis.

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