Automated Liquid Handling
Automated Liquid Handling

Chlamydia Trachomatis Diagnostic Kit (PCR-Fluorescence Probing)

Chlamydia trachomatis is a small obligate intracellular bacterial pathogen commonly spread as a sexually transmitted infection. The C. trachomatis PCR diagnostic test kit is designed to detect and quantify these pathogens and provides the broadest possible detection profile so as to ensure that all clinically relevant subtypes are detected. Detailed clinical study has validated the high specificity, sensitivity, and reproducibility of the kit with analytical sensitivity of 1000 copies/mL in the sample. The kit contains reagents for nucleic acid extraction, realtime-PCR detection and quality control.

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Catalogue #: ABI - Chlamydia Trachomatis Diagnostic Kit

Principles of the test

The kit has specifically designed primers as well as a fluorescent probe thereby allowing utilization of realtime fluorescent PCR. The genetic sequence of C. trachomatis can be targeted through realtime-PCR amplification. Variation in the fluorescent signal is directly proportional to the quantity of amplified product.

Key Features

The kit contains ready-to-use reagents, offers fast real-time detection and provides excellent sensitivity and high specificity. It is also optimized for use in automated liquid handling workstations. By combining this kit with Aurora’s VERSA™ workstations, laboratories can fully automate the amplification process thereby reducing cost and operator error, while increasing efficiency.

Product Features:

  • 48 tests per kit (25 µL reaction)
  • Rapid detection of C. trachomatis
  • All necessary PCR reagents pre-mixed and ready-to-go
  • Kit applicable for most commercially available realtime PCR instruments
  • High sensitivity and high precision in determining virus types
  • Standard instrument set-up parameters
  • Accurate controls to confirm findings
  • Colour coded reagent vials

Standard Kit Contents:

  • DNA extraction reagent
  • PCR detection reagent
    • PCR Buffer
    • Primer/Probe/dNTPs
    • Taq mixture
  • MgCl2
  • Quality control reagent
    • C. trachomatis positive control
    • C. trachomatis quantification reference
    • Negative control
  • Diluent

Dynamic range of kit

Under optimal PCR conditions the kit has very high priming efficiencies of >95% and can detect between 1X108 and 1X103 copies of target template.

Handling & Storage Guidelines

Kit Storage & Shelf life - Store at -20ºC±5ºC. Avoid repeated freezing and thawing. The kit is stable until the expiry date shown.

Sample Requirements & Capabilities

Endocervical and urethral swab specimens. This test is not intended for use with ocular secretion, throat, rectal or other types of specimens.

Sample collection, storage and transportation

Please follow the current guidelines and ordinance on the Chlamydia trachomatis specimen collection, handling, storage, packaging, transport requirements.

Disclaimer: The kit is for detection of the Chlamydia trachomatis in endocervical and urethral swab specimens and may be used for the laboratory diagnosis and monitoring the particular virus infection. The kit is for research only, and should not be used in clinical diagnosis.

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